abstract：:A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been established for the rapid and reliable determination of gamma-aminobutyric acid (GABA) and glutamate in brain microdialysates. The microdialysis samples were analysed using a HILIC (hydrophilic interaction liquid chromatography) column, which ...

journal_title：Journal of neuroscience methods

authors： Buck K,Voehringer P,Ferger B

更新日期：2009-08-30 00:00:00

abstract：:The GABA-A receptor plays a critical role in inhibitory neurotransmission in the brain. Quantitation of GABA-A receptor subunits in various brain regions is essential to understand their role in plasticity and brain disorders. However, conventional RNA assays are tedious and less sensitive for use in studies of subuni...

journal_title：Journal of neuroscience methods

authors： Gangisetty O,Reddy DS

更新日期：2009-06-30 00:00:00

abstract：:We have designed a device for long-term head fixation for use in behaving nonhuman primates that is robust yet minimally invasive and simple to use. This device is a modified version of the halo system that is used in humans for cervical traction and stabilization after spinal column injuries. This device consists of ...

journal_title：Journal of neuroscience methods

authors： Davis TS,Torab K,House P,Greger B

更新日期：2009-06-30 00:00:00

abstract：:Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...

journal_title：Journal of neuroscience methods

authors： Deighton RF,Short DM,McGregor RJ,Gow AJ,Whittle IR,McCulloch J

更新日期：2009-06-15 00:00:00

abstract：:Subcortical white matter stroke is a common stroke subtype but has had limited pre-clinical modeling. Recapitulating this disease process in mice has been impeded by the relative inaccessibility of the subcortical white matter arterial supply to induce white matter ischemia in isolation. In this report, we detail a su...

journal_title：Journal of neuroscience methods

authors： Sozmen EG,Kolekar A,Havton LA,Carmichael ST

更新日期：2009-06-15 00:00:00

abstract：:Non-invasive neuroimaging is increasingly used for investigating the human amygdala. Accurate functional localization in the amygdala region is, however, challenging and quantitative data on the anatomical specificity of functional amygdala imaging is lacking. We have therefore retrospectively investigated 114 recentl...

journal_title：Journal of neuroscience methods

authors： Ball T,Derix J,Wentlandt J,Wieckhorst B,Speck O,Schulze-Bonhage A,Mutschler I

更新日期：2009-05-30 00:00:00

abstract：:To obtain a high quality EMG acquisition, the signal must be recorded as far away as possible from muscle innervations and tendon zones, which are known to shift during dynamic contractions. This study describes a methodology, using commercial bipolar electrodes, to identify better electrode positions for superficial ...

journal_title：Journal of neuroscience methods

authors： Sacco IC,Gomes AA,Otuzi ME,Pripas D,Onodera AN

更新日期：2009-05-30 00:00:00

abstract：:Ketamine, a non-competitive N-methyl-D-aspartate (NMDA) antagonist, is a commonly used injectable anaesthetic agent. In the present study, ketamine- and isoflurane-induced anaesthesias were tested to identify the influence of different anaesthesia methods in conjunction with the unilateral quinolinic acid-induced exci...

journal_title：Journal of neuroscience methods

authors： Jiang W,Büchele F,Papazoglou A,Döbrössy M,Nikkhah G

更新日期：2009-05-15 00:00:00

abstract：:Nitric oxide (NO) is a gas messenger with diverse physiological roles in the nervous system, from modulation of synaptic plasticity and neurogenesis to the mediation of neuronal death. NO production in the brain is catalyzed by three isoforms of NO synthase (NOS) including neuronal NOS (nNOS), inducible NOS and endoth...

journal_title：Journal of neuroscience methods

authors： Mahairaki V,Xu L,Farah MH,Hatfield G,Kizana E,Marbán E,Koliatsos VE

更新日期：2009-05-15 00:00:00

abstract：:This study examined the effect of two dissection techniques on the quality of human brain specimens. Frozen cerebellar samples were obtained from postmortem brains of 10 subjects free from neurological and psychiatric disease. These tissues were tested for RNA and DNA concentration and quality after being dissected wi...

journal_title：Journal of neuroscience methods

authors： Buerlein RC,Hyde TM,Lipska BK,Robinson W,Khosla A,Kleinman JE

更新日期：2009-04-30 00:00:00

abstract：:Bone marrow stroma cells-derived neural stem cells (BMSCs-D-NSCs) transplantation is a promising strategy for the treatment of nervous system disorders. The development of a non-invasive method to follow the fate of BMSCs-D-NSCs in vivo is very important for the future application of this treatment. In this paper, we ...

journal_title：Journal of neuroscience methods

authors： Ke YQ,Hu CC,Jiang XD,Yang ZJ,Zhang HW,Ji HM,Zhou LY,Cai YQ,Qin LS,Xu RX

更新日期：2009-04-30 00:00:00

abstract：:The detection and characterization of bursting activity remains a topic where no consensual definition has been reached so far. We compare here three different approaches of spike trains variability: statistical characterization (average frequency, coefficient of variation), burst detection (Poisson and rank surprise)...

journal_title：Journal of neuroscience methods

authors： Robin K,Maurice N,Degos B,Deniau JM,Martinerie J,Pezard L

更新日期：2009-04-30 00:00:00

abstract：:Parkinson's disease (PD) exhibits symptoms of motor dysfunction such as tremor, akinesia and rigidity. Agents that selectively disrupt or destroy catecholaminergic systems, such as reserpine, methamphetamine, 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, have been used to develop PD models and to...

journal_title：Journal of neuroscience methods

authors： Luthra PM,Barodia SK,Raghubir R

更新日期：2009-04-15 00:00:00

abstract：:The development and effective use of robust high-electrode-count microelectrode arrays for neuronal recording and stimulation depends on effective monitoring of electrode impedances and how these change over time. In multielectrode arrays, conventional electrode impedance measurements may be confounded by possible shu...

journal_title：Journal of neuroscience methods

authors： Gunalan K,Warren DJ,Perry JD,Normann RA,Clark GA

更新日期：2009-04-15 00:00:00

abstract：:Extracellular electrical stimulation is increasingly used for in vitro neural experimentation, including brain slices and cultured cells. Although it is desirable to record directly from the stimulating electrode, relatively high stimulation levels make it extremely difficult to record immediately after the stimulatio...

journal_title：Journal of neuroscience methods

authors： Nam Y,Brown EA,Ross JD,Blum RA,Wheeler BC,DeWeerth SP

更新日期：2009-03-30 00:00:00

abstract：:A method for quantifying nociceptive withdrawal reflex receptive fields in human volunteers and patients is described. The reflex receptive field (RRF) for a specific muscle denotes the cutaneous area from which a muscle contraction can be evoked by a nociceptive stimulus. The method is based on random stimulations pr...

journal_title：Journal of neuroscience methods

authors： Neziri AY,Curatolo M,Bergadano A,Petersen-Felix S,Dickenson A,Arendt-Nielsen L,Andersen OK

更新日期：2009-03-30 00:00:00

abstract：:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...

journal_title：Journal of neuroscience methods

authors： Alper BJ,Schmidt WK

更新日期：2009-03-30 00:00:00

abstract：:Alzheimer's disease (AD) is a progressive degenerative disorder of the brain characterized by a slow, progressive decline in cognitive function and behavior. As the disease advances, persons have a tough time with daily tasks like using the phone, cooking, handling money or driving the car. AD affects 15 million peopl...

journal_title：Journal of neuroscience methods

authors： Wilson B,Samanta MK,Santhi K,Sampath Kumar KP,Ramasamy M,Suresh B

更新日期：2009-03-15 00:00:00

abstract：:Electroporation creates transient pores in the plasma membrane to introduce macromolecules within a cell or cell population. Generally, electrical pulses are delivered between two electrodes separated from each other, making electroporation less likely to be localised. We have developed a new device combining local pr...

journal_title：Journal of neuroscience methods

authors： Barker M,Billups B,Hamann M

更新日期：2009-03-15 00:00:00

abstract：:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...

journal_title：Journal of neuroscience methods

authors： Kondra S,Laishram J,Ban J,Migliorini E,Di Foggia V,Lazzarino M,Torre V,Ruaro ME

更新日期：2009-02-15 00:00:00

abstract：:In order to identify novel genes involved in appetite and body weight regulation we have developed a microarray based method suitable for detecting small changes in gene expression in discrete groups of hypothalamic neurons. The method is based on a combination of stereological sampling, laser capture microdissection ...

journal_title：Journal of neuroscience methods

authors： Paulsen SJ,Larsen LK,Jelsing J,Janssen U,Gerstmayer B,Vrang N

更新日期：2009-02-15 00:00:00

abstract：:The study of dendritic spine shape and number has become a standard in the analysis of synaptic transmission anomalies since a considerable number of neuropsychiatric and neurological diseases have their foundation in alterations in these structures. One of the best ways to study possible alterations of dendritic spin...

journal_title：Journal of neuroscience methods

authors： Melendez-Ferro M,Perez-Costas E,Roberts RC

更新日期：2009-01-30 00:00:00

abstract：:Neuromuscular electrical stimulation (NMES) can provide functional movements in people after central nervous system injury. The neuroplastic effects of long-term NMES-induced repetitive limb movement are not well understood. A rodent model of neurotrauma in which NMES can be implemented may be effective for such inves...

journal_title：Journal of neuroscience methods

authors： Ichihara K,Venkatasubramanian G,Abbas JJ,Jung R

更新日期：2009-01-30 00:00:00

abstract：:Spinal muscular atrophy (SMA), the leading genetic cause of infant death results from loss of spinal motor neurons causing atrophy of skeletal muscle. SMA is caused by loss of the Survival Motor Neuron 1 (SMN1) gene, however, an identically coding gene called SMN2 is retained, but is alternatively spliced to produce a...

journal_title：Journal of neuroscience methods

authors： Mattis VB,Butchbach ME,Lorson CL

更新日期：2008-10-30 00:00:00

abstract：:We assessed the potential of using multielectrode arrays (MEAs) to investigate several physiological properties of the calyx of Held synapse in the medial nucleus of the trapezoid body of gerbil. Due to the large size of the synapse, it became widely employed in studies on synaptic mechanisms. Electrical stimulation a...

journal_title：Journal of neuroscience methods

authors： Haustein MD,Reinert T,Warnatsch A,Englitz B,Dietz B,Robitzki A,Rübsamen R,Milenkovic I

更新日期：2008-09-30 00:00:00

abstract：:The ability to detect and sort overlapping spike waveforms in extracellular recordings is key to studies of neural coding at high spatial and temporal resolution. Most spike-sorting algorithms are based on initial spike detection (e.g. by a voltage threshold) and subsequent waveform classification. Much effort has bee...

journal_title：Journal of neuroscience methods

authors： Herbst JA,Gammeter S,Ferrero D,Hahnloser RH

更新日期：2008-09-15 00:00:00

abstract：:Songbirds are the preeminent animal model for understanding how the brain encodes and produces learned vocalizations. Here, we report a new statistical method, the Kullback-Leibler (K-L) distance, for analyzing vocal change over time. First, we use a computerized recording system to capture all song syllables produced...

journal_title：Journal of neuroscience methods

authors： Wu W,Thompson JA,Bertram R,Johnson F

更新日期：2008-09-15 00:00:00

abstract：:Implantable microfabricated microelectrode arrays represent a versatile and powerful tool to record electrophysiological activity across multiple spatial locations in the brain. Spikes and field potentials, however, correspond to only a fraction of the physiological information available at the neural interface. In ur...

journal_title：Journal of neuroscience methods

authors： Johnson MD,Franklin RK,Gibson MD,Brown RB,Kipke DR

更新日期：2008-09-15 00:00:00

abstract：:Features extracted with optimized wavelets were compared with standard methods for a Brain-Computer Interface driven by non-motor imagery tasks. Two non-motor imagery tasks were used, Auditory Imagery of a familiar tune and Spatial Navigation Imagery through a familiar environment. The aims of this study were to evalu...

journal_title：Journal of neuroscience methods

authors： Cabrera AF,Dremstrup K

更新日期：2008-09-15 00:00:00

abstract：:Prepulse inhibition (PPI) of the acoustic startle response is a measure of sensory motor gating, and is affected in various neuropsychiatric disorders. Although PPI has been used extensively to study both the neural effects of such conditions, as well as in the search for animal models, a number of critical issues hav...

journal_title：Journal of neuroscience methods

authors： Adams AL,Hudson A,Ryan CL,Doucette TA

更新日期：2008-08-30 00:00:00

abstract：:Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not s...

journal_title：Journal of neuroscience methods

authors： Harris JE,Friedland JS

更新日期：2008-08-30 00:00:00

abstract：:Neuroendocrine cells like chromaffin cells and PC-12 cells are established models for transport, docking and secretion of secretory vesicles. In micrographs, these vesicles are recognized by their electron dense core. The analysis of secretory vesicle distribution is usually performed manually, which is labour-intensi...

journal_title：Journal of neuroscience methods

authors： van Weering JR,Wijntjes R,de Wit H,Wortel J,Cornelisse LN,Veldkamp WJ,Verhage M

更新日期：2008-08-15 00:00:00

abstract：:Rapid prototyping (RP) is a useful method for designing and fabricating a wide variety of devices used for neuroscience research. The present study confirms the utility of using fused deposition modeling, a specific form of RP, to produce three devices commonly used for basic science experimentation. The accuracy and ...

journal_title：Journal of neuroscience methods

authors： Tek P,Chiganos TC,Mohammed JS,Eddington DT,Fall CP,Ifft P,Rousche PJ

更新日期：2008-07-30 00:00:00

abstract：:Many behaviors of interest to neurophysiologists are difficult to study under laboratory conditions because such behaviors are often inhibited when an animal is restrained and socially isolated. Even under the best conditions, such behaviors may be sparse enough as to require long duration neural recordings or simulta...

journal_title：Journal of neuroscience methods

authors： Eliades SJ,Wang X

更新日期：2008-07-30 00:00:00

abstract：:The objective of the present work was to examine fronto-central spindle frequency. A previously validated spindle detector, providing an electroencephalographic (EEG) amplitude independent spindle detection, was used to detect bilateral sleep spindles from sleep EEG recordings of ten healthy subjects with a time resol...

journal_title：Journal of neuroscience methods

authors： Huupponen E,Kulkas A,Tenhunen M,Saastamoinen A,Hasan J,Himanen SL

更新日期：2008-07-15 00:00:00

abstract：:N-Acetylaspartate (NAA) is an important marker of neuronal function and viability that can be measured using magnetic resonance spectroscopy (MRS). In this paper, we proposed a method to measure NAA synthesis using proton MRS with infusion of uniformly (13)C-labeled glucose, and demonstrated its feasibility in an in v...

journal_title：Journal of neuroscience methods

authors： Xu S,Yang J,Shen J

更新日期：2008-07-15 00:00:00

abstract：:Brain diffusion properties are at present most commonly evaluated by magnetic resonance (MR) diffusion imaging. MR cannot easily distinguish between the extracellular and intracellular signal components, but the older technique of real-time iontophoresis (RTI) detects exclusively extracellular diffusion. Interpretatio...

journal_title：Journal of neuroscience methods

authors： Kaur G,Hrabetova S,Guilfoyle DN,Nicholson C,Hrabe J

更新日期：2008-06-30 00:00:00

abstract：:A current source for neural stimulation is presented which converts arbitrary voltage signals to current-controlled signals while regulating the offset-voltage across the stimulation electrodes in order to keep the electrodes in an electrochemical state that allows for injecting a maximum charge. The offset-voltage ca...

journal_title：Journal of neuroscience methods

authors： Schuettler M,Franke M,Krueger TB,Stieglitz T

更新日期：2008-06-30 00:00:00

abstract：:Foraging is a rapid, side-to-side movement of the nose generated by Caenorhabditis elegans as it explores its environment. In this paper, we present an automated method to detect and analyze foraging behavior of C. elegans in a video sequence. Several morphological image-processing methods are used to locate the preci...

journal_title：Journal of neuroscience methods

authors： Huang KM,Cosman P,Schafer WR

更新日期：2008-06-15 00:00:00

abstract：:Mutant protein aggregates are an important biomarker in Huntington's and other neurodegenerative diseases however their quantification has typically relied on manual imaging and counting, or cell-free assays, which do not allow for concurrent analysis of cell viability. Here we describe four automated high throughput ...

journal_title：Journal of neuroscience methods

authors： Scotter EL,Narayan P,Glass M,Dragunow M

更新日期：2008-06-15 00:00:00